A Method for the Immortalization of Newborn Mouse Skin Keratinocytes
نویسندگان
چکیده
منابع مشابه
A Method for the Immortalization of Newborn Mouse Skin Keratinocytes
Isolation and culture of mouse primary epidermal keratinocytes is a common technique that allows for easy genetic and environmental manipulation. However, due to their limited lifespan in culture, experiments utilizing primary keratinocytes require large numbers of animals, and are time consuming and expensive. To avoid these issues, we developed a method for the immortalization of primary mous...
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As a major component of the epidermal tissue, a primary keratinocyte has served as an essential tool not only for the study of pathogenesis of skin-related diseases but also for the assessment of potential toxicities of various chemicals used in cosmetics. However, its short lifespan in ex vivo setting has been a great hurdle for many practical applications. Therefore, a number of immortalizati...
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Skin cancer has been the most usual and illustrates 50% of all new cancers detected each year. If they detected at an early stage, treatment can become simple and economically. Accurate skin lesion segmentation is important in automated early skin cancer detection and diagnosis systems. The aim of this study is to provide an effective approach to detect the skin lesion border on a purposed imag...
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ژورنال
عنوان ژورنال: Frontiers in Oncology
سال: 2015
ISSN: 2234-943X
DOI: 10.3389/fonc.2015.00177